A vaginal cannula is used to introduce the ozone gas to the patient. Unlike Rectal Insufflation, there is no risk of pressure buildup. The ozone concentration used is usually 25-30ug/ml, and the flow rate is usually 1/4LPM (liters per minute) or 1/8LPM. Treatment time is usually 5 – 15 minutes. The patient may lower the ozone concentration, and the duration of the treatment if any discomfort occurs. This treatment should not be performed more than once per day, and is usually only performed 2-3 times per week.